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Western blot All lanes: NME6 antibody at 2microg/ml + Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 22, 14, 19 kDa Observed band size: 22 kDa
Western blot All lanes: NME6 antibody at 2microg/ml + Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 22, 14, 19 kDa Observed band size: 22 kDa
Western blot All lanes: NME6 antibody at 2microg/ml + Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 22, 14, 19 kDa Observed band size: 22 kDa

NME6 Antibody

CSB-PA015891LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetNME6
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Overview

  • Supplier
    Cusabio
  • Product Name
    NME6 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10201
  • Target name
    NME6
  • Target description
    NME/NM23 nucleoside diphosphate kinase 6
  • Target synonyms
    inhibitor of p53-induced apoptosis-alpha; IPIA-ALPHA; NDK 6; NDP kinase 6; NM23-H6; non-metastatic cells 6, protein expressed in (nucleoside-diphosphate kinase); nucleoside diphosphate kinase 6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75414
  • Protein Name
    Nucleoside diphosphate kinase 6
  • Scientific Description
    Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Inhibitor of p53-induced apoptosis.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NME6 using anti-NME6 antibody (A10922-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Lncap whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NME6 antigen affinity purified polyclonal antibody (Catalog # A10922-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NME6 at approximately 21 kDa. The expected band size for NME6 is at 21 kDa.
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