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Anti-NOD1 Antibody

144-62342
RayBiotech
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetNOD1
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-NOD1 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Conjugate
    Unconjugated
  • Gene ID10392
  • Target name
    NOD1
  • Target description
    nucleotide binding oligomerization domain containing 1
  • Target synonyms
    CARD4; caspase recruitment domain family, member 4; caspase recruitment domain-containing protein 4; CLR7.1; NLR family, CARD domain containing 1; NLRC1; nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 1; nucleotide-binding oligomerization domain-containing protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y239
  • Protein Name
    Nucleotide-binding oligomerization domain-containing protein 1
  • Scientific Description
    NOD1 pAb
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CARD4/NOD1 using anti-CARD4/NOD1 antibody (A00495-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: monkey heart tissue lysates, Lane 2: monkey skeletal muscle tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CARD4/NOD1 antigen affinity purified polyclonal antibody (Catalog # A00495-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CARD4/NOD1 at approximately 108KD. The expected band size for CARD4/NOD1 is at 108KD.
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