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Immunofluorescent staining of human cell line HaCaT shows localization to mitochondria.
Immunofluorescent staining of human cell line HaCaT shows localization to mitochondria.
Immunofluorescent staining of human cell line HaCaT shows localization to mitochondria.

Anti-NOD1 Antibody

HPA074367
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetNOD1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NOD1 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10392
  • Target name
    NOD1
  • Target description
    nucleotide binding oligomerization domain containing 1
  • Target synonyms
    CARD4, CLR7.1, NLRC1, hNod1, nucleotide-binding oligomerization domain-containing protein 1, NLR family, CARD domain containing 1, caspase recruitment domain family, member 4, caspase recruitment domain-containing protein 4, nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y239
  • Protein Name
    Nucleotide-binding oligomerization domain-containing protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Lane 1: Raw264.7 cell lysates; Lane 2: Mouse thymus lysates probed with CARD4 Polyclonal Antibody, Unconjugated (bs-7085R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of CARD4/NOD1 using anti-CARD4/NOD1 antibody (A00495-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: monkey heart tissue lysates, Lane 2: monkey skeletal muscle tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CARD4/NOD1 antigen affinity purified polyclonal antibody (Catalog # A00495-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CARD4/NOD1 at approximately 108KD. The expected band size for CARD4/NOD1 is at 108KD.
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