Figure 1. Western blot analysis of NOLA1/GAR1 using anti-NOLA1/GAR1 antibody (A07049-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A375 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOLA1/GAR1 antigen affinity purified polyclonal antibody (Catalog # A07049-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOLA1/GAR1 at approximately 25 kDa. The expected band size for NOLA1/GAR1 is at 25 kDa.
Figure 1. Western blot analysis of NOLA1/GAR1 using anti-NOLA1/GAR1 antibody (A07049-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A375 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOLA1/GAR1 antigen affinity purified polyclonal antibody (Catalog # A07049-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOLA1/GAR1 at approximately 25 kDa. The expected band size for NOLA1/GAR1 is at 25 kDa.
Anti-NOLA1/GAR1 Antibody Picoband(r)
A07049-1-HRP
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetGAR1
Overview
- SupplierBoster Bio
- Product NameAnti-NOLA1/GAR1 Antibody Picoband(r)
- Delivery Days Customer9
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateHRP
- Gene ID54433
- Target nameGAR1
- Target descriptionGAR1 ribonucleoprotein
- Target synonymsGAR1 homolog, ribonucleoprotein; GAR1 ribonucleoprotein homolog; H/ACA ribonucleoprotein complex subunit 1; NOLA1; nucleolar protein family A member 1; nucleolar protein family A, member 1 (H/ACA small nucleolar RNPs); snoRNP protein GAR1
- HostRabbit
- IsotypeIgG
- Protein IDQ9NY12
- Protein NameH/ACA ribonucleoprotein complex subunit 1
- Scientific DescriptionBoster Bio Anti-NOLA1/GAR1 Antibody Picoband® catalog # A07049-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203