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Immunohistochemical staining of human skin shows strong positivity in nucleoli in squamous epithelial cells.
Immunohistochemical staining of human skin shows strong positivity in nucleoli in squamous epithelial cells.
Immunohistochemical staining of human skin shows strong positivity in nucleoli in squamous epithelial cells.

Anti-NOP2 Antibody

HPA040119
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNOP2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NOP2 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4839
  • Target name
    NOP2
  • Target description
    NOP2 nucleolar protein
  • Target synonyms
    NOL1, NOP120, NSUN1, p120, 28S rRNA (cytosine(4447)-C(5))-methyltransferase, NOL1/NOP2/Sun domain family, member 1, NOP2 nucleolar protein homolog, nucleolar protein 1, 120kDa, nucleolar protein 2 homolog, probable 28S rRNA (cytosine(4447)-C(5))-methyltransferase, proliferating-cell nucleolar antigen p120, proliferation-associated nucleolar protein p120, putative ribosomal RNA methyltransferase NOP2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP46087
  • Protein Name
    28S rRNA (cytosine(4447)-C(5))-methyltransferase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of NOP2 using anti-NOP2 antibody (A04830-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human RT4 whole cell lysates, Lane 6: human 293T tissue lysates, Lane 7: human SIHA whole cell lyates, Lane 8: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOP2 antigen affinity purified polyclonal antibody (Catalog # A04830-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOP2 at approximately 120 kDa. The expected band size for NOP2 is at 120 kDa.
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