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NOP2 Polyclonal Antibody

BS-9710R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Equine, Human, Mouse, Rabbit, Rat, Sheep
TargetNOP2
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Overview

  • Supplier
    Bioss
  • Product Name
    NOP2 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400), IF(IHC-P)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID4839
  • Target name
    NOP2
  • Target description
    NOP2 nucleolar protein
  • Target synonyms
    NOL1, NOP120, NSUN1, p120, 28S rRNA (cytosine(4447)-C(5))-methyltransferase, NOL1/NOP2/Sun domain family, member 1, NOP2 nucleolar protein homolog, nucleolar protein 1, 120kDa, nucleolar protein 2 homolog, probable 28S rRNA (cytosine(4447)-C(5))-methyltransferase, proliferating-cell nucleolar antigen p120, proliferation-associated nucleolar protein p120, putative ribosomal RNA methyltransferase NOP2
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Bovine, Canine, Equine, Human, Mouse, Rabbit, Rat, Sheep
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NOP2 using anti-NOP2 antibody (A04830-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human RT4 whole cell lysates, Lane 6: human 293T tissue lysates, Lane 7: human SIHA whole cell lyates, Lane 8: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOP2 antigen affinity purified polyclonal antibody (Catalog # A04830-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOP2 at approximately 120 kDa. The expected band size for NOP2 is at 120 kDa.
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