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Immunohistochemical staining of human cerebral cortex shows moderate nuclear positivity.
Immunohistochemical staining of human cerebral cortex shows moderate nuclear positivity.
Immunohistochemical staining of human cerebral cortex shows moderate nuclear positivity.

Anti-NPAS2 Antibody

HPA019674
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNPAS2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NPAS2
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4862
  • Target name
    NPAS2
  • Target description
    neuronal PAS domain protein 2
  • Target synonyms
    MOP4, PASD4, bHLHe9, neuronal PAS domain-containing protein 2, PAS domain-containing protein 4, basic-helix-loop-helix-PAS protein MOP4, class E basic helix-loop-helix protein 9, member of PAS protein 4, member of PAS superfamily 4, neuronal PAS2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ99743
  • Protein Name
    Neuronal PAS domain-containing protein 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of NPAS2 using anti-NPAS2 antibody (A02688-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysate,Lane 2: mouse brain tissue lysate,Lane 3: human COLO-320 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NPAS2 antigen affinity purified polyclonal antibody (Catalog # A02688-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NPAS2 at approximately 92KD. The expected band size for NPAS2 is at 92KD.
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