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Western Blot analysis of rat stomach 293 cells using NPAS2 Polyclonal Antibody
Western Blot analysis of rat stomach 293 cells using NPAS2 Polyclonal Antibody
Western Blot analysis of rat stomach 293 cells using NPAS2 Polyclonal Antibody

NPAS2 Antibody

CSB-PA004658
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Rat
TargetNPAS2
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Overview

  • Supplier
    Cusabio
  • Product Name
    NPAS2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4862
  • Target name
    NPAS2
  • Target description
    neuronal PAS domain protein 2
  • Target synonyms
    basic-helix-loop-helix-PAS protein MOP4; bHLHe9; class E basic helix-loop-helix protein 9; member of PAS protein 4; member of PAS superfamily 4; MOP4; neuronal PAS domain-containing protein 2; neuronal PAS2; PAS domain-containing protein 4; PASD4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ99743
  • Protein Name
    Neuronal PAS domain-containing protein 2
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NPAS2 using anti-NPAS2 antibody (A02688-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysate,Lane 2: mouse brain tissue lysate,Lane 3: human COLO-320 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NPAS2 antigen affinity purified polyclonal antibody (Catalog # A02688-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NPAS2 at approximately 92KD. The expected band size for NPAS2 is at 92KD.
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