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Anti-NPLOC4 Antibody

144-03256
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetNPLOC4
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-NPLOC4 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55666
  • Target name
    NPLOC4
  • Target description
    NPL4 homolog, ubiquitin recognition factor
  • Target synonyms
    NPL4, nuclear protein localization protein 4 homolog, NPLOC4 ubiquitin recognition factor, nuclear protein localization 4 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8TAT6
  • Protein Name
    Nuclear protein localization protein 4 homolog
  • Scientific Description
    NPLOC4 Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NPL4/NPLOC4 using anti-NPL4/NPLOC4 antibody (A08476-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NPL4/NPLOC4 antigen affinity purified polyclonal antibody (Catalog # A08476-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NPL4/NPLOC4 at approximately 72 kDa. The expected band size for NPL4/NPLOC4 is at 68 kDa.
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