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Immunohistochemical staining of human testis shows strong granular cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.
Immunohistochemical staining of human testis shows strong granular cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.
Immunohistochemical staining of human testis shows strong granular cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.

Anti-NPLOC4 Antibody

HPA021560
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetNPLOC4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NPLOC4
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55666
  • Target name
    NPLOC4
  • Target description
    NPL4 homolog, ubiquitin recognition factor
  • Target synonyms
    NPL4; NPLOC4 ubiquitin recognition factor; nuclear protein localization 4 homolog; nuclear protein localization protein 4 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8TAT6
  • Protein Name
    Nuclear protein localization protein 4 homolog
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NPL4/NPLOC4 using anti-NPL4/NPLOC4 antibody (A08476-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NPL4/NPLOC4 antigen affinity purified polyclonal antibody (Catalog # A08476-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NPL4/NPLOC4 at approximately 72 kDa. The expected band size for NPL4/NPLOC4 is at 68 kDa.
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Immunohistochemistry analysis in human testis and pancreas tissues using Anti-NPLOC4 antibody. Corresponding NPLOC4 RNA-seq data are presented for the same tissues.
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