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Immunofluorescent staining of human cell line HaCaT shows localization to nuclear bodies.
Immunofluorescent staining of human cell line HaCaT shows localization to nuclear bodies.
Immunofluorescent staining of human cell line HaCaT shows localization to nuclear bodies.

Anti-NR1D1 Antibody

HPA007935
Atlas Antibodies
ApplicationsImmunoCytoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetNR1D1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NR1D1
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9572
  • Target name
    NR1D1
  • Target description
    nuclear receptor subfamily 1 group D member 1
  • Target synonyms
    EAR1; ear-1; hRev; nuclear receptor Rev-ErbA-alpha; nuclear receptor subfamily 1 group D member 1; REVERBA; Rev-ErbAalpha; rev-erbA-alpha; REVERBalpha; THRA1; THRAL; V-erbA-related protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP20393
  • Protein Name
    Nuclear receptor subfamily 1 group D member 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of KDM1B using anti-KDM1B antibody (M01077-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: human U2OS whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KDM1B antigen affinity purified monoclonal antibody (Catalog # M01077-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KDM1B at approximately 55 kDa. The expected band size for KDM1B is at 67 kDa.
Product group Antibodies
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