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Immunohistochemical staining of human heart muscle, lymphoid tissues, placenta and skeletal muscle using Anti-NRAP antibody HPA037954 (A) shows similar protein distribution across tissues to independent antibody HPA037953 (B).
Immunohistochemical staining of human heart muscle, lymphoid tissues, placenta and skeletal muscle using Anti-NRAP antibody HPA037954 (A) shows similar protein distribution across tissues to independent antibody HPA037953 (B).
Immunohistochemical staining of human heart muscle, lymphoid tissues, placenta and skeletal muscle using Anti-NRAP antibody HPA037954 (A) shows similar protein distribution across tissues to independent antibody HPA037953 (B).

Anti-NRAP Antibody

HPA037954
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNRAP
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NRAP
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4892
  • Target name
    NRAP
  • Target description
    nebulin related anchoring protein
  • Target synonyms
    nebulin-related-anchoring protein; N-RAP
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ86VF7
  • Protein Name
    Nebulin-related-anchoring protein
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of NRAP using anti-NRAP antibody (A09321-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat H9C2 whole cell lysates, Lane 2: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NRAP antigen affinity purified polyclonal antibody (Catalog # A09321-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NRAP at approximately 250 kDa. The expected band size for NRAP is at 197 kDa.
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Immunohistochemical staining of human heart muscle, lymphoid tissues, placenta and skeletal muscle using Anti-NRAP antibody HPA037953 (A) shows similar protein distribution across tissues to independent antibody HPA037954 (B).
Product group Antibodies
Atlas Antibodies
Anti-NRAP AntibodyHPA037953
ApplicationsImmunoHistoChemistry
ReactivityHuman
TargetNRAP
  • SizePrice
Atlas Antibodies
PrEST Antigen NRAPAPREST80406
Protein IDQ86VF7
  • SizePrice