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NRAP Antibody

CAB12813
Assay Genie
TargetNRAP
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    NRAP Antibody
  • Delivery Days Customer
    9
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID4892
  • Target name
    NRAP
  • Target description
    nebulin related anchoring protein
  • Target synonyms
    N-RAP, nebulin-related-anchoring protein
  • Host
    Rabbit
  • Protein IDQ86VF7
  • Protein Name
    Nebulin-related-anchoring protein
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NRAP using anti-NRAP antibody (A09321-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat H9C2 whole cell lysates, Lane 2: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NRAP antigen affinity purified polyclonal antibody (Catalog # A09321-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NRAP at approximately 250 kDa. The expected band size for NRAP is at 197 kDa.
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Immunohistochemical staining of human heart muscle, lymphoid tissues, placenta and skeletal muscle using Anti-NRAP antibody HPA037954 (A) shows similar protein distribution across tissues to independent antibody HPA037953 (B).
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