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Immunohistochemical staining of human placenta shows moderate cytoplasmic and nuclear positivity in decidual cells.
Immunohistochemical staining of human placenta shows moderate cytoplasmic and nuclear positivity in decidual cells.
Immunohistochemical staining of human placenta shows moderate cytoplasmic and nuclear positivity in decidual cells.

Anti-NRBF2 Antibody

HPA021670
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetNRBF2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NRBF2
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID29982
  • Target name
    NRBF2
  • Target description
    nuclear receptor binding factor 2
  • Target synonyms
    comodulator of PPAR and RXR 1; comodulator of PPAR and RXR 2; COPR; COPR1; COPR2; NRBF-2; nuclear receptor-binding factor 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96F24
  • Protein Name
    Nuclear receptor-binding factor 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NRBF2 using anti-NRBF2 antibody (A09144-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human U-87MG whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NRBF2 antigen affinity purified polyclonal antibody (Catalog # A09144-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NRBF2 at approximately 38 kDa. The expected band size for NRBF2 is at 32 kDa.
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