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Western Blot analysis of A549 cells using NRBF-2 Polyclonal Antibody
Western Blot analysis of A549 cells using NRBF-2 Polyclonal Antibody
Western Blot analysis of A549 cells using NRBF-2 Polyclonal Antibody

NRBF2 Antibody

CSB-PA003479
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNRBF2
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Overview

  • Supplier
    Cusabio
  • Product Name
    NRBF2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID29982
  • Target name
    NRBF2
  • Target description
    nuclear receptor binding factor 2
  • Target synonyms
    comodulator of PPAR and RXR 1; comodulator of PPAR and RXR 2; COPR; COPR1; COPR2; NRBF-2; nuclear receptor-binding factor 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96F24
  • Protein Name
    Nuclear receptor-binding factor 2
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NRBF2 using anti-NRBF2 antibody (A09144-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human U-87MG whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NRBF2 antigen affinity purified polyclonal antibody (Catalog # A09144-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NRBF2 at approximately 38 kDa. The expected band size for NRBF2 is at 32 kDa.
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