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Immunohistochemistry analysis in human testis and liver tissues using AMAb90848 antibody. Corresponding NSD2 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human testis and liver tissues using AMAb90848 antibody. Corresponding NSD2 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human testis and liver tissues using AMAb90848 antibody. Corresponding NSD2 RNA-seq data are presented for the same tissues.

Anti-NSD2 Antibody

AMAB90848
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNSD2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NSD2 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CL1057
  • Conjugate
    Unconjugated
  • Gene ID7468
  • Target name
    NSD2
  • Target description
    nuclear receptor binding SET domain protein 2
  • Target synonyms
    KMT3F, KMT3G, MMSET, RAUST, REIIBP, TRX5, WHS, WHSC1, histone-lysine N-methyltransferase NSD2, IL5 promoter REII region-binding protein, Wolf-Hirschhorn syndrome candidate 1, multiple myeloma SET domain containing protein type III, nuclear SET domain-containing protein 2, probable histone-lysine N-methyltransferase NSD2, trithorax/ash1-related protein 5
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDO96028
  • Protein Name
    Histone-lysine N-methyltransferase NSD2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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IHC image of CSB-PA026107LA01HU diluted at 1:400 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Figure 1. Western blot analysis of WHSC1/NSD2 using anti-WHSC1/NSD2 antibody (A32441-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: human HEK293 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WHSC1/NSD2 antigen affinity purified polyclonal antibody (Catalog # A32441-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WHSC1/NSD2 at approximately 70 kDa, 160 kDa. The expected band size for WHSC1/NSD2 is at 152 kDa.
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