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WHSC1 / NSD2 Antibody

LS-C409485
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetNSD2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    WHSC1 / NSD2 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 30kDa/53kDa/66kDa/69kDa/71kDa/80kDa/152kDa, while the observed MW by Western blot was 80kDa/152kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 30kDa/53kDa/66kDa/69kDa/71kDa/80kDa/152kDa, while the observed MW by Western blot was 80kDa/152kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.81 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID7468
  • Target name
    NSD2
  • Target description
    nuclear receptor binding SET domain protein 2
  • Target synonyms
    histone-lysine N-methyltransferase NSD2; IL5 promoter REII region-binding protein; KMT3F; KMT3G; MMSET; multiple myeloma SET domain containing protein type III; nuclear SET domain-containing protein 2; probable histone-lysine N-methyltransferase NSD2; REIIBP; trithorax/ash1-related protein 5; TRX5; WHS; WHSC1; Wolf-Hirschhorn syndrome candidate 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of WHSC1/NSD2 using anti-WHSC1/NSD2 antibody (A32441-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: human HEK293 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WHSC1/NSD2 antigen affinity purified polyclonal antibody (Catalog # A32441-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WHSC1/NSD2 at approximately 70 kDa, 160 kDa. The expected band size for WHSC1/NSD2 is at 152 kDa.
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Immunohistochemistry analysis in human testis and liver tissues using AMAb90848 antibody. Corresponding NSD2 RNA-seq data are presented for the same tissues.
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