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Immunohistochemical staining of human urinary bladder shows strong nuclear positivity in urothelial cells.
Immunohistochemical staining of human urinary bladder shows strong nuclear positivity in urothelial cells.
Immunohistochemical staining of human urinary bladder shows strong nuclear positivity in urothelial cells.

Anti-NSMCE4A Antibody

HPA037459
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNSMCE4A
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NSMCE4A
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54780
  • Target name
    NSMCE4A
  • Target description
    NSE4 homolog A, SMC5-SMC6 complex component
  • Target synonyms
    C10orf86; non-SMC element 4 homolog A; non-structural maintenance of chromosomes element 4 homolog A; NS4EA; NSE4 homolog, SMC5-SMC6 complex component A; NSE4A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NXX6
  • Protein Name
    Non-structural maintenance of chromosomes element 4 homolog A
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NSMCE4A using anti-NSMCE4A antibody (A14289-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat ovary tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse ovary tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NSMCE4A antigen affinity purified polyclonal antibody (Catalog # A14289-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NSMCE4A at approximately 50 kDa. The expected band size for NSMCE4A is at 44 kDa.
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