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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.

Anti-NSMCE4A Antibody

HPA044872
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetNSMCE4A
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NSMCE4A Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54780
  • Target name
    NSMCE4A
  • Target description
    NSE4A component of SMC5/6 complex
  • Target synonyms
    C10orf86, NS4EA, NSE4A, non-structural maintenance of chromosomes element 4 homolog A, NSE4 homolog A, SMC5-SMC6 complex component, NSE4 homolog, SMC5-SMC6 complex component A, non-SMC element 4 homolog A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NXX6
  • Protein Name
    Non-structural maintenance of chromosomes element 4 homolog A
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of NSMCE4A using anti-NSMCE4A antibody (A14289-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat ovary tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse ovary tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NSMCE4A antigen affinity purified polyclonal antibody (Catalog # A14289-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NSMCE4A at approximately 50 kDa. The expected band size for NSMCE4A is at 44 kDa.
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