Figure 1. Western blot analysis of NSUN3 using anti-NSUN3 antibody (A16002). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NSUN3 antigen affinity purified polyclonal antibody (Catalog # A16002) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NSUN3 at approximately 38 kDa. The expected band size for NSUN3 is at 38 kDa.
Figure 1. Western blot analysis of NSUN3 using anti-NSUN3 antibody (A16002). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NSUN3 antigen affinity purified polyclonal antibody (Catalog # A16002) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NSUN3 at approximately 38 kDa. The expected band size for NSUN3 is at 38 kDa.
Anti-NSUN3 Antibody Picoband(r)
A16002-CY3
ApplicationsFlow Cytometry, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Monkey
TargetNSUN3
Overview
- SupplierBoster Bio
- Product NameAnti-NSUN3 Antibody Picoband(r)
- Delivery Days Customer9
- Application Supplier NoteTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsFlow Cytometry, Western Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateCy3
- Gene ID63899
- Target nameNSUN3
- Target descriptionNOP2/Sun RNA methyltransferase 3
- Target synonymsCOXPD48; MST077; MSTP077; NOL1/NOP2/Sun domain family member 3; NOP2/Sun RNA methyltransferase family member 3; putative methyltransferase NSUN3; tRNA (cytosine(34)-C(5))-methyltransferase, mitochondrial
- HostRabbit
- IsotypeIgG
- Protein IDQ9H649
- Protein NametRNA (cytosine(34)-C(5))-methyltransferase, mitochondrial
- Scientific DescriptionBoster Bio Anti-NSUN3 Antibody Picoband® catalog # A16002. Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Monkey
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203