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Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.

Anti-NSUN4 Antibody

HPA028489
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetNSUN4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NSUN4
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID387338
  • Target name
    NSUN4
  • Target description
    NOP2/Sun RNA methyltransferase 4
  • Target synonyms
    5-methylcytosine rRNA methyltransferase NSUN4; 5-methylcytosine tRNA methyltransferase NSUN4; NOL1/NOP2/Sun domain family member 4; NOP2/Sun domain family, member 4; NOP2/Sun RNA methyltransferase family member 4; putative methyltransferase NSUN4; SHTAP; sperm head and tail associated protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96CB9
  • Protein Name
    5-methylcytosine rRNA methyltransferase NSUN4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NSUN4 using anti-NSUN4 antibody (A09446-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NSUN4 antigen affinity purified polyclonal antibody (Catalog # A09446-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NSUN4 at approximately 40 kDa. The expected band size for NSUN4 is at 43 kDa.
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