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Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.

Anti-NSUN4 Antibody

HPA028489
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNSUN4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NSUN4 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID387338
  • Target name
    NSUN4
  • Target description
    NOP2/Sun RNA methyltransferase 4
  • Target synonyms
    SHTAP, 5-cytosine rRNA methyltransferase NSUN4, 5-cytosine tRNA methyltransferase NSUN4, 5-methylcytosine rRNA methyltransferase NSUN4, 5-methylcytosine tRNA methyltransferase NSUN4, NOL1/NOP2/Sun domain family member 4, NOP2/Sun RNA methyltransferase family member 4, NOP2/Sun domain family, member 4, mRNA cytosine C(5)-methyltransferase NSUN4, putative methyltransferase NSUN4, sperm head and tail associated protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96CB9
  • Protein Name
    5-cytosine rRNA methyltransferase NSUN4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NSUN4 antibody [N1C2] (GTX120442) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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Figure 1. Western blot analysis of NSUN4 using anti-NSUN4 antibody (A09446-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NSUN4 antigen affinity purified polyclonal antibody (Catalog # A09446-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NSUN4 at approximately 40 kDa. The expected band size for NSUN4 is at 43 kDa.
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