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Nsun4 Polyclonal Antibody

CAC09629
Biomatik
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetNSUN4
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Overview

  • Supplier
    Biomatik
  • Product Name
    Nsun4 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, WB, IHC; Recommended dilution: WB:1:500-2000, IHC:1:20-1:200
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID387338
  • Target name
    NSUN4
  • Target description
    NOP2/Sun RNA methyltransferase 4
  • Target synonyms
    SHTAP, 5-cytosine rRNA methyltransferase NSUN4, 5-cytosine tRNA methyltransferase NSUN4, 5-methylcytosine rRNA methyltransferase NSUN4, 5-methylcytosine tRNA methyltransferase NSUN4, NOL1/NOP2/Sun domain family member 4, NOP2/Sun RNA methyltransferase family member 4, NOP2/Sun domain family, member 4, mRNA cytosine C(5)-methyltransferase NSUN4, putative methyltransferase NSUN4, sperm head and tail associated protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96CB9
  • Protein Name
    5-cytosine rRNA methyltransferase NSUN4
  • Scientific Description
    The Nsun4 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NSUN4 antibody [N1C2] (GTX120442) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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Figure 1. Western blot analysis of NSUN4 using anti-NSUN4 antibody (A09446-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NSUN4 antigen affinity purified polyclonal antibody (Catalog # A09446-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NSUN4 at approximately 40 kDa. The expected band size for NSUN4 is at 43 kDa.
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