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Anti-NSUN6 Antibody

Anti-NSUN6 Antibody

A39670
Antibodies.com
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityHuman
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Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-NSUN6 Antibody
  • Delivery Days Customer
    7
  • Applications
    ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Scientific Description
    Rabbit polyclonal antibody to NSUN6
  • Reactivity
    Human
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NSUN6 using anti-NSUN6 antibody (A13404-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NSUN6 antigen affinity purified polyclonal antibody (A13404-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NSUN6 at approximately 52 kDa. The expected band size for NSUN6 is at 52 kDa.
Product group Antibodies
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Anti-NSUN6 Antibody Picoband(r)A13404-3-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetNSUN6
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Product group Antibodies
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ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse
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Product group Antibodies
LifeSpan BioSciences
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ApplicationsImmunoFluorescence, Western Blot
ReactivityHuman, Mouse
TargetNSUN6
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Immunohistochemical staining of human Liver shows strong granular cytoplasmic positivity in hepatocytes.
Product group Antibodies
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ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetNSUN6
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Product group Antibodies
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Formalin-fixed and paraffin embedded human breast cancer labeled with Anti-NSUN6 Polyclonal Antibody, Unconjugated (bs-19484R) at 1:500 followed by conjugation to the secondary antibody and DAB staining.
Product group Antibodies
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NSUN6 Polyclonal AntibodyBS-19484R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetNSUN6
  • SizePrice
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NSUN6 antibody [N3C3] (GTX107157) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
GeneTex
NSUN6 antibody [N3C3]GTX107157
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman
TargetNSUN6
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