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Immunohistochemical staining of human skin shows weak to moderate cytoplasmic positivity in squamous epithelial cells.
Immunohistochemical staining of human skin shows weak to moderate cytoplasmic positivity in squamous epithelial cells.
Immunohistochemical staining of human skin shows weak to moderate cytoplasmic positivity in squamous epithelial cells.

Anti-NTN3 Antibody

HPA050817
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNTN3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NTN3 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4917
  • Target name
    NTN3
  • Target description
    netrin 3
  • Target synonyms
    NTN2L, netrin-3, Netrin-2, chicken, homolog of, like, netrin-2-like protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00634
  • Protein Name
    Netrin-3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of NTN3 using anti-NTN3 antibody (A17692). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NTN3 antigen affinity purified polyclonal antibody (Catalog # A17692) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NTN3 at approximately 70 kDa. The expected band size for NTN3 is at 70 kDa.
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TargetNTN3
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