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ProductsBack/NTN3 / NTN2L Antibody (aa431-459)

NTN3 / NTN2L Antibody (aa431-459)

LS-C163036
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetNTN3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NTN3 / NTN2L Antibody (aa431-459)
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4917
  • Target name
    NTN3
  • Target description
    netrin 3
  • Target synonyms
    NTN2L, netrin-3, Netrin-2, chicken, homolog of, like, netrin-2-like protein
  • Host
    Rabbit
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NTN3 using anti-NTN3 antibody (A17692). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NTN3 antigen affinity purified polyclonal antibody (Catalog # A17692) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NTN3 at approximately 70 kDa. The expected band size for NTN3 is at 70 kDa.
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ReactivityHuman, Rat
TargetNTN3
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