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Immunohistochemical staining of human kidney shows strong cytoplasmic and membranous positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic and membranous positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic and membranous positivity in cells in tubules.

Anti-NUDT6 Antibody

HPA039202
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNUDT6
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NUDT6
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID11162
  • Target name
    NUDT6
  • Target description
    nudix hydrolase 6
  • Target synonyms
    ASFGF2, FGF-AS, FGF2AS, GFG-1, GFG1, nucleoside diphosphate-linked moiety X motif 6, antisense basic fibroblast growth factor, nudix (nucleoside diphosphate linked moiety X)-type motif 6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP53370
  • Protein Name
    Nucleoside diphosphate-linked moiety X motif 6
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of NUDT6 using anti-NUDT6 antibody (A08301-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human A549 whole cell lysates, Lane 7: human Hela whole cell lysates, Lane 8: human SH-SY5Y whole cell lysates, Lane 9: rat kidney tissue lysates, Lane 10: rat H9C2 whole cell lysates, Lane 11: mouse kidney tissue lysates, Lane 12: mouse HEPA1/6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUDT6 antigen affinity purified polyclonal antibody (Catalog # A08301-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUDT6 at approximately 72 kDa. The expected band size for NUDT6 is at 72 kDa.
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