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Anti-NUDT6

Y058352
Applied Biological Materials
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
100 ul
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Overview

  • Supplier
    Applied Biological Materials
  • Product Name
    Anti-NUDT6
  • Delivery Days Customer
    9
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    This gene overlaps and lies on the opposite strand from FGF2 gene, and is thought to be the FGF2 antisense gene. The two genes are independently transcribed, and their expression shows an inverse relationship, suggesting that this antisense transcript may regulate FGF2 expression. This gene has also been shown to have hormone-regulatory and antiproliferative actions in the pituitary that are independent of FGF2 expression. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NUDT6 using anti-NUDT6 antibody (A08301-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human A549 whole cell lysates, Lane 7: human Hela whole cell lysates, Lane 8: human SH-SY5Y whole cell lysates, Lane 9: rat kidney tissue lysates, Lane 10: rat H9C2 whole cell lysates, Lane 11: mouse kidney tissue lysates, Lane 12: mouse HEPA1/6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUDT6 antigen affinity purified polyclonal antibody (Catalog # A08301-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUDT6 at approximately 72 kDa. The expected band size for NUDT6 is at 72 kDa.
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