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Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in granular pattern in tubular cells.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in granular pattern in tubular cells.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in granular pattern in tubular cells.

Anti-NUDT7 Antibody

HPA042042
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetNUDT7
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NUDT7
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID283927
  • Target name
    NUDT7
  • Target description
    nudix hydrolase 7
  • Target synonyms
    nudix (nucleoside diphosphate linked moiety X)-type motif 7; peroxisomal coenzyme A diphosphatase NUDT7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP0C024
  • Protein Name
    Peroxisomal coenzyme A diphosphatase NUDT7
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NUDT7 using anti-NUDT7 antibody (A10663-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUDT7 antigen affinity purified polyclonal antibody (A10663-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUDT7 at approximately 27 kDa. The expected band size for NUDT7 is at 27 kDa.
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