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NUDT7 Antibody

CSB-PA890440XA01DOA
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityPlant
TargetNUDT7
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Overview

  • Supplier
    Cusabio
  • Product Name
    NUDT7 Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID826884
  • Target name
    NUDT7
  • Target description
    MutT/nudix family protein
  • Target synonyms
    AT4G12720, ATNUDX7, Arabidopsis thaliana Nudix hydrolase homolog 7, AtNUDT7, GFG1, GROWTH FACTOR GENE 1, T20K18.70, T20K18_70, MutT/nudix family protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9SU14
  • Protein Name
    Nudix hydrolase 7
  • Reactivity
    Plant
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NUDT7 using anti-NUDT7 antibody (A10663-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUDT7 antigen affinity purified polyclonal antibody (A10663-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUDT7 at approximately 27 kDa. The expected band size for NUDT7 is at 27 kDa.
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