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Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in glial cells.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in glial cells.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in glial cells.

Anti-NUMBL Antibody

HPA058380
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNUMBL
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NUMBL Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9253
  • Target name
    NUMBL
  • Target description
    NUMB like endocytic adaptor protein
  • Target synonyms
    CAG3A, CTG3a, NBL, NUMB-R, NUMBLIKE, NUMBR, TNRC23, numb-like protein, numb homolog-like, numb-related protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y6R0
  • Protein Name
    Numb-like protein
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in a few neuronal cells.
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IHC image of CSB-PA897595LA01HU diluted at 1:300 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Figure 1. Western blot analysis of NUMBL using anti-NUMBL antibody (A07925). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U-87 MG whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUMBL antigen affinity purified polyclonal antibody (Catalog # A07925) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUMBL at approximately 72 kDa. The expected band size for NUMBL is at 65-70 kDa.
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