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ProductsBack/NUMBLIKE / NUMBL Antibody

NUMBLIKE / NUMBL Antibody

LS-C749098
LifeSpan BioSciences
ApplicationsImmunoHistoChemistry
Product group Antibodies
TargetNUMBL
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NUMBLIKE / NUMBL Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 64kDa.
  • Applications
    ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:50 - 1:200) The predicted MW is 64kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID9253
  • Target name
    NUMBL
  • Target description
    NUMB like endocytic adaptor protein
  • Target synonyms
    CAG3A; CTG3a; NBL; numb homolog-like; NUMBLIKE; numb-like protein; NUMBR; NUMB-R; numb-related protein; TNRC23
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NUMBL using anti-NUMBL antibody (A07925). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U-87 MG whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUMBL antigen affinity purified polyclonal antibody (Catalog # A07925) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUMBL at approximately 72 kDa. The expected band size for NUMBL is at 65-70 kDa.
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