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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus.
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus.
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus.

Anti-NUP214 Antibody

HPA048789
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetNUP214
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NUP214 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8021
  • Target name
    NUP214
  • Target description
    nucleoporin 214
  • Target synonyms
    CAIN, CAN, IIAE9, nuclear pore complex protein Nup214, CAN protein, putative oncogene, nucleoporin 214kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35658
  • Protein Name
    Nuclear pore complex protein Nup214
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NUP214 using anti-NUP214 antibody (A02408). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human THP-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUP214 antigen affinity purified polyclonal antibody (Catalog # A02408) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUP214 at approximately 280KD. The expected band size for NUP214 is at 214KD.
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