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ProductsBack/NUP214 / CAN Antibody

NUP214 / CAN Antibody

LS-C409893
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetNUP214
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NUP214 / CAN Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 212kDa/213kDa/215kDa, while the observed MW by Western blot was 280kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 212kDa/213kDa/215kDa, while the observed MW by Western blot was 280kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8021
  • Target name
    NUP214
  • Target description
    nucleoporin 214
  • Target synonyms
    CAIN; CAN; CAN protein, putative oncogene; IIAE9; nuclear pore complex protein Nup214; nucleoporin 214kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NUP214 using anti-NUP214 antibody (A02408). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human THP-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUP214 antigen affinity purified polyclonal antibody (Catalog # A02408) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUP214 at approximately 280KD. The expected band size for NUP214 is at 214KD.
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