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Anti-OGG1 Antibody

144-62942
RayBiotech
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetOGG1
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-OGG1 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Conjugate
    Unconjugated
  • Gene ID4968
  • Target name
    OGG1
  • Target description
    8-oxoguanine DNA glycosylase
  • Target synonyms
    8-hydroxyguanine DNA glycosylase; AP lyase; DNA-apurinic or apyrimidinic site lyase; HMMH; HOGG1; MUTM; N-glycosylase/DNA lyase; OGG1 type 1f; OGH1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15527
  • Protein Name
    N-glycosylase/DNA lyase
  • Scientific Description
    OGG1 pAb
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Ogg1 using anti-Ogg1 antibody (A00768-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysate, Lane 2: rat thymus tissue lysate, Lane 3: rat liver tissue lysate, Lane 4: rat testis tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ogg1 antigen affinity purified polyclonal antibody (Catalog # A00768-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ogg1 at approximately 39KD. The expected band size for Ogg1 is at 39KD.
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Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with OGG1 Polyclonal Antibody, Unconjugated (bs-3687R) at 1:200 overnight at 4°C, DAB staining.
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