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Immunohistochemistry analysis in human testis and prostate tissues using HPA052271 antibody. Corresponding OIP5 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human testis and prostate tissues using HPA052271 antibody. Corresponding OIP5 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human testis and prostate tissues using HPA052271 antibody. Corresponding OIP5 RNA-seq data are presented for the same tissues.

Anti-OIP5 Antibody

HPA052271
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetOIP5
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-OIP5 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID11339
  • Target name
    OIP5
  • Target description
    Opa interacting protein 5
  • Target synonyms
    5730547N13Rik, CT86, LINT-25, MIS18B, MIS18beta, hMIS18beta, protein Mis18-beta, LAP2alpha interactor-25, MIS18 kinetochore protein homolog B, cancer/testis antigen 86, opa-interacting protein 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43482
  • Protein Name
    Protein Mis18-beta
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Immunohistochemistry analysis in human testis and prostate tissues using Anti-OIP5 antibody. Corresponding OIP5 RNA-seq data are presented for the same tissues.
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Figure 1. Western blot analysis of OIP5 using anti-OIP5 antibody (A07819). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OIP5 antigen affinity purified polyclonal antibody (Catalog # A07819) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OIP5 at approximately 22 kDa. The expected band size for OIP5 is at 25 kDa.
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