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OIP5 Antibody

LS-C396817
LifeSpan BioSciences
ApplicationsELISA
Product group Antibodies
ReactivityHuman
TargetOIP5
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    OIP5 Antibody
  • Delivery Days Customer
    23
  • Applications
    ELISA
  • Applications Supplier
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID11339
  • Target name
    OIP5
  • Target description
    Opa interacting protein 5
  • Target synonyms
    5730547N13Rik, CT86, LINT-25, MIS18B, MIS18beta, hMIS18beta, protein Mis18-beta, LAP2alpha interactor-25, MIS18 kinetochore protein homolog B, cancer/testis antigen 86, opa-interacting protein 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C,-80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of OIP5 using anti-OIP5 antibody (A07819). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OIP5 antigen affinity purified polyclonal antibody (Catalog # A07819) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OIP5 at approximately 22 kDa. The expected band size for OIP5 is at 25 kDa.
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