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Immunohistochemical staining of human gallbladder shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human gallbladder shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human gallbladder shows moderate cytoplasmic positivity in glandular cells.

Anti-OLFML2B Antibody

HPA054136
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetOLFML2B
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-OLFML2B Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID25903
  • Target name
    OLFML2B
  • Target description
    olfactomedin like 2B
  • Target synonyms
    olfactomedin-like protein 2B, photomedin-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ68BL8
  • Protein Name
    Olfactomedin-like protein 2B
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of OLFML2B using anti-OLFML2B antibody (A16481-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OLFML2B antigen affinity purified polyclonal antibody (Catalog # A16481-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OLFML2B at approximately 96 kDa. The expected band size for OLFML2B is at 84 kDa.
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