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WB analysis of HUVEC cell lysates using GTX87915 OLFML2B antibody. The lane on the right is blocked with the synthesized peptide.
WB analysis of HUVEC cell lysates using GTX87915 OLFML2B antibody. The lane on the right is blocked with the synthesized peptide.
WB analysis of HUVEC cell lysates using GTX87915 OLFML2B antibody. The lane on the right is blocked with the synthesized peptide.

OLFML2B antibody

GTX87915
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetOLFML2B
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Overview

  • Supplier
    GeneTex
  • Product Name
    OLFML2B antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500~1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID25903
  • Target name
    OLFML2B
  • Target description
    olfactomedin like 2B
  • Target synonyms
    olfactomedin-like protein 2B, photomedin-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ68BL8
  • Protein Name
    Olfactomedin-like protein 2B
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of OLFML2B using anti-OLFML2B antibody (A16481-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OLFML2B antigen affinity purified polyclonal antibody (Catalog # A16481-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OLFML2B at approximately 96 kDa. The expected band size for OLFML2B is at 84 kDa.
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