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Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm.
Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm.
Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm.

Anti-ONECUT2 Antibody

HPA057058
Atlas Antibodies
ApplicationsChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetONECUT2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ONECUT2 Antibody
  • Delivery Days Customer
    4
  • Applications
    ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9480
  • Target name
    ONECUT2
  • Target description
    one cut homeobox 2
  • Target synonyms
    OC-2, OC2, one cut domain family member 2, HNF-6-beta, ONECUT-2 homeodomain transcription factor, hepatocyte nuclear factor 6-beta, onecut 2, transcription factor ONECUT-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95948
  • Protein Name
    One cut domain family member 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of ONECUT2 using anti-ONECUT2 antibody (A09652-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human HepG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ONECUT2 antigen affinity purified polyclonal antibody (Catalog # A09652-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ONECUT2 at approximately 54KD. The expected band size for ONECUT2 is at 54KD.
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ReactivityHuman, Mouse
TargetONECUT2
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Atlas Antibodies
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Product group Antibodies
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Onecut2 Polyclonal AntibodyCAC07875
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TargetONECUT2
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Mouse cerebrum lysates probed with ONECUT2 Polyclonal Antibody, Unconjugated (bs-19643R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C.
Product group Antibodies
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ONECUT2 Polyclonal AntibodyBS-19643R
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ReactivityHuman, Mouse, Rat
TargetONECUT2
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Western Blot Positive WB detected in: A549 whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate All lanes: ONECUT2 antibody at 2.4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 55 kDa Observed band size: 55 kDa
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ONECUT2 AntibodyCSB-PA016337LA01HU
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TargetONECUT2
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ONECUT2 / OC2 Antibody (FITC)LS-C499461
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TargetONECUT2
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ONECUT2 AntibodyPACO49834
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