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Western Blot Positive WB detected in: A549 whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate All lanes: ONECUT2 antibody at 2.4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 55 kDa Observed band size: 55 kDa
Western Blot Positive WB detected in: A549 whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate All lanes: ONECUT2 antibody at 2.4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 55 kDa Observed band size: 55 kDa
Western Blot Positive WB detected in: A549 whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate All lanes: ONECUT2 antibody at 2.4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 55 kDa Observed band size: 55 kDa

ONECUT2 Antibody

CSB-PA016337LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetONECUT2
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Overview

  • Supplier
    Cusabio
  • Product Name
    ONECUT2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9480
  • Target name
    ONECUT2
  • Target description
    one cut homeobox 2
  • Target synonyms
    OC-2, OC2, one cut domain family member 2, HNF-6-beta, ONECUT-2 homeodomain transcription factor, hepatocyte nuclear factor 6-beta, onecut 2, transcription factor ONECUT-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95948
  • Protein Name
    One cut domain family member 2
  • Scientific Description
    Transcriptional activator. Activates the transcription of a number of liver genes such as HNF3B.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ONECUT2 using anti-ONECUT2 antibody (A09652-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human HepG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ONECUT2 antigen affinity purified polyclonal antibody (Catalog # A09652-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ONECUT2 at approximately 54KD. The expected band size for ONECUT2 is at 54KD.
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