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Immunohistochemical staining of human parathyroid gland shows cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human parathyroid gland shows cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human parathyroid gland shows cytoplasmic positivity in glandular cells.

Anti-OPA3 Antibody

HPA044881
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetOPA3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-OPA3
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID80207
  • Target name
    OPA3
  • Target description
    outer mitochondrial membrane lipid metabolism regulator OPA3
  • Target synonyms
    3-methylglutaconic aciduria type III; MGA3; OPA3 outer mitochondrial membrane lipid metabolism regulator; optic atrophy 3 (autosomal recessive, with chorea and spastic paraplegia); Optic atrophy 3 (Iraqi-Jewish 'optic atrophy plus'); optic atrophy 3 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H6K4
  • Protein Name
    Optic atrophy 3 protein
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of OPA3 using anti-OPA3 antibody (A06345-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OPA3 antigen affinity purified polyclonal antibody (Catalog # A06345-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OPA3 at approximately 20 kDa. The expected band size for OPA3 is at 20 kDa.
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