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Anti-OPA3 Antibody

CAB7997
Assay Genie
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetOPA3
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Overview

  • Supplier
    Assay Genie
  • Product Name
    Anti-OPA3 Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    Not applicable
  • Conjugate
    Unconjugated
  • Gene ID80207
  • Target name
    OPA3
  • Target description
    outer mitochondrial membrane lipid metabolism regulator OPA3
  • Target synonyms
    MGA3, optic atrophy 3 protein, 3-methylglutaconic aciduria type III, OPA3 outer mitochondrial membrane lipid metabolism regulator, Optic atrophy 3 (Iraqi-Jewish 'optic atrophy plus'), optic atrophy 3 (autosomal recessive, with chorea and spastic paraplegia)
  • Host
    Rabbit
  • Protein IDQ9H6K4
  • Protein Name
    Optic atrophy 3 protein
  • Scientific Description
    OPA3 Antibody is a premium polyclonal that offers outstanding performance and reliability for demanding research applications. Rigorously validated for WB, ELISA, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Human,Mouse,Rat samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 100microL format to meet your experimental needs. For optimal performance, store at -20°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity
    Human
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of OPA3 using anti-OPA3 antibody (A06345-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OPA3 antigen affinity purified polyclonal antibody (Catalog # A06345-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OPA3 at approximately 20 kDa. The expected band size for OPA3 is at 20 kDa.
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