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Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.

Anti-ORAI2 Antibody

HPA055137
Atlas Antibodies
ApplicationsImmunoCytoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetORAI2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ORAI2
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID80228
  • Target name
    ORAI2
  • Target description
    ORAI calcium release-activated calcium modulator 2
  • Target synonyms
    C7orf19; CAP-binding protein complex interacting protein 2; CBCIP2; H_NH0514P08.8; MEM142B; protein orai-2; putative protein ORAI2-2; TMEM142B; transmembrane protein 142B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96SN7
  • Protein Name
    Protein orai-2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Orai2 using anti-Orai2 antibody (A07411-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human A375 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Orai2 antigen affinity purified polyclonal antibody (Catalog # A07411-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Orai2 at approximately 39KD. The expected band size for Orai2 is at 39KD.
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