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Immunohistochemical staining of human duodenum shows cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human duodenum shows cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human duodenum shows cytoplasmic positivity in glandular cells.

Anti-OSBPL2 Antibody

HPA041127
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetOSBPL2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-OSBPL2
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9885
  • Target name
    OSBPL2
  • Target description
    oxysterol binding protein like 2
  • Target synonyms
    DFNA67; DNFA67; ORP2; ORP-2; OSBP-related protein 2; oxysterol-binding protein-related protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H1P3
  • Protein Name
    Oxysterol-binding protein-related protein 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Western blot All lanes: OSBPL2 antibody at 1ug/ml Lane 1: HepG2 whole cell lysate Lane 2: Mouse heart tissue Lane 3: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 56, 54 kDa Observed band size: 56 kDa
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Immunohistochemical staining of human duodenum shows cytoplasmic positivity in glandular cells.
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Figure 1. Western blot analysis of OSBPL2 using anti-OSBPL2 antibody (A07543-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OSBPL2 antigen affinity purified polyclonal antibody (Catalog # A07543-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OSBPL2 at approximately 55 kDa. The expected band size for OSBPL2 is at 55 kDa.
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