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ProductsBack/OSBPL2 Antibody
Western blot All lanes: OSBPL2 antibody at 1ug/ml Lane 1: HepG2 whole cell lysate Lane 2: Mouse heart tissue Lane 3: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 56, 54 kDa Observed band size: 56 kDa
Western blot All lanes: OSBPL2 antibody at 1ug/ml Lane 1: HepG2 whole cell lysate Lane 2: Mouse heart tissue Lane 3: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 56, 54 kDa Observed band size: 56 kDa
Western blot All lanes: OSBPL2 antibody at 1ug/ml Lane 1: HepG2 whole cell lysate Lane 2: Mouse heart tissue Lane 3: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 56, 54 kDa Observed band size: 56 kDa

OSBPL2 Antibody

CSB-PA884442LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetOSBPL2
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Overview

  • Supplier
    Cusabio
  • Product Name
    OSBPL2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9885
  • Target name
    OSBPL2
  • Target description
    oxysterol binding protein like 2
  • Target synonyms
    DFNA67; DNFA67; ORP2; ORP-2; OSBP-related protein 2; oxysterol-binding protein-related protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H1P3
  • Protein Name
    Oxysterol-binding protein-related protein 2
  • Scientific Description
    Binds phospholipids; exhibits strong binding to phosphatidic acid and weak binding to phosphatidylinositol 3-phosphate (PubMed:11279184). Binds 25-hydroxycholesterol (PubMed:17428193).
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of OSBPL2 using anti-OSBPL2 antibody (A07543-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OSBPL2 antigen affinity purified polyclonal antibody (Catalog # A07543-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OSBPL2 at approximately 55 kDa. The expected band size for OSBPL2 is at 55 kDa.
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