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Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.
Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.
Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.

Anti-OSBPL9 Antibody

HPA027378
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetOSBPL9
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-OSBPL9 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID114883
  • Target name
    OSBPL9
  • Target description
    oxysterol binding protein like 9
  • Target synonyms
    ORP-9, ORP9, oxysterol-binding protein-related protein 9, OSBP-related protein 9
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96SU4
  • Protein Name
    Oxysterol-binding protein-related protein 9
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of OSBPL9 using anti-OSBPL9 antibody (A09498-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OSBPL9 antigen affinity purified polyclonal antibody (Catalog # A09498-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OSBPL9 at approximately 95 kDa. The expected band size for OSBPL9 is at 90 kDa.
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