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Anti-Osteocalcin [KTM219]

AB02244-1.1-BT
Absolute Antibody
ApplicationsELISA
Product group Antibodies
TargetBGLAP
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-Osteocalcin [KTM219]
  • Delivery Days Customer
    9
  • Application Supplier Note
    This antibody is recommended for detection of low moecular weight peptides by using open sandwich ELISA (OS-ELISA). This antibody detects C-terminal of human osteocalcin in an ELISA. The cloned VH/VL cDNA fragments of this antibody were successful in detecting C-terminal peptides as short as 6 residues in an OS-ELISA. (PMID: 17636882). The VH-MBP and VL-MBP fusion proteins of this antibody were also used in the detection of small biomarker peptide by a micro OS-ELISA integrated into a microfluidic device (PMID: 20024056). The affinity matured version of this antibody O2AG2 was also made using OS-ELISA. It is also available on request (PMID: 20123883). This antibody was also used in the development of a highly sensitive protein based open sandwich immuno-PCR for detection of small biomarkers (PMID: 24025570)
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    KTM219
  • Gene ID632
  • Target name
    BGLAP
  • Target description
    bone gamma-carboxyglutamate protein
  • Target synonyms
    BGP; bone gamma-carboxyglutamate (gla) protein (osteocalcin); gamma-carboxyglutamic acid-containing protein; OC; OCN; osteocalcin
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP02818
  • Protein Name
    Osteocalcin
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Osteocalcin Polyclonal Antibody(bs-0470R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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