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Osteocalcin Antibody

ABX024044
Abbexa
ApplicationsCLIA ChemiLuminecent ImmunoAssay, ELISA, RadioImmunoAssay
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Product group Antibodies
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Overview

  • Supplier
    Abbexa
  • Product Name
    Osteocalcin Antibody
  • Delivery Days Customer
    12
  • Applications
    CLIA ChemiLuminecent ImmunoAssay, ELISA, RadioImmunoAssay
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Host
    Mouse
  • UNSPSC
    12352203

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Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Osteocalcin Polyclonal Antibody(bs-0470R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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Figure 1. IHC analysis of Osteocalcin using anti-Osteocalcin antibody (PB9918). Osteocalcin was detected in a paraffin-embedded section of human osteosarcoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 microg/ml rabbit anti-Osteocalcin Antibody (PB9918) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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