More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Anti-osteopontin [hu1A12]

Anti-osteopontin [hu1A12]

AB00750-1.1-BT
Absolute Antibody
ApplicationsELISA, ImmunoHistoChemistry, Neutralisation/Blocking, Other Application
Product group Antibodies
TargetSPP1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-osteopontin [hu1A12]
  • Delivery Days Customer
    9
  • Application Supplier Note
    hu1A12 suppreses MDA-MB-435S (highly metastatic human breast cancer cell line) cell migration (evaluated by scratch-wound healing) and also inhibits cell adhesion of MDA-MB-435S cells to human OPN. hu1A12 also inhibits the anti-apoptotic and pro-survival functions of OPN possibly through upregulating Bax, downregulating Bcl-2, and blocking the activation of NF-kappaB. hu1A12 also has been shown to suppress primary breast tumour growth in humans through in vivo studies. This antibody has also been used in ELISA, IHC and SPR studies.
  • Applications
    ELISA, ImmunoHistoChemistry, Neutralisation/Blocking, Other Application
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    hu1A12
  • Gene ID6696
  • Target name
    SPP1
  • Target description
    secreted phosphoprotein 1
  • Target synonyms
    BNSP, BSPI, ETA-1, OPN, osteopontin, SPP1/CALPHA1 fusion, early T-lymphocyte activation 1, lnc-PKD2-2-3, nephropontin, osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein, secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1), urinary stone protein, uropontin
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP10451
  • Protein Name
    Osteopontin
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

FAQ

Go to our frequently asked questions

Related products

Western Blot analysis of 453 cells using OPN Polyclonal Antibody
Product group Antibodies
Cusabio
SPP1 AntibodyCSB-PA003599
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSPP1
  • SizePrice
Product group Antibodies
Everest Biotech
Goat anti-Osteopontin / SPP1EB06825
ApplicationsWestern Blot, ELISA
TargetSPP1
  • SizePrice
Product group Antibodies
MedChem Express
Osteopontin Antibody(Rabbit)HY-P80771
TargetSPP1
  • SizePrice
Anti-Osteopontin Antibody
Product group Antibodies
Antibodies.com
Anti-Osteopontin AntibodyA84105
ApplicationsWestern Blot, ELISA
  • SizePrice
Figure 1. Western blot analysis of Osteopontin using anti-Osteopontin antibody (RP1080). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: human SHG-44 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Osteopontin antigen affinity purified polyclonal antibody (Catalog # RP1080) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Osteopontin at approximately 66,25-55KD. The expected band size for Osteopontin is at 35KD.
Product group Antibodies
Boster Bio
Anti-Osteopontin/SPP1 Antibody Picoband(r)RP1080-CARRIER-FREE
ApplicationsWestern Blot, ELISA
TargetSPP1
  • SizePrice
Product group Antibodies
RayBiotech
Anti-Mouse SPP1 Antibody144-01361
ApplicationsWestern Blot
TargetSPP1
  • SizePrice
Product group Antibodies
Nordic-MUbio
Chicken anti human osteopontin-c (the shortest splice variant of osteopontin)AhOPNc
ApplicationsWestern Blot, ELISA
TargetSPP1
  • SizePrice
Immunohistochemistry analysis in human cerebral cortex and tonsil tissues using AMAb91653 antibody. Corresponding SPP1 RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
Anti-SPP1 AntibodyAMAB91653
ApplicationsImmunoHistoChemistry
ReactivityHuman
TargetSPP1
  • SizePrice
Mouse kidney lysates probed with Osteopontin Polyclonal Antibody, unconjugated (bs-0019R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.
Product group Antibodies
References
Bioss
Osteopontin Polyclonal AntibodyBS-0019R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetSPP1
  • SizePrice
Product group Antibodies
References
GeneTex
Osteopontin antibodyGTX132742
ApplicationsWestern Blot
TargetSPP1
  • SizePrice