Bio-Connect
Mouse kidney lysates probed with Osteopontin Polyclonal Antibody, unconjugated (bs-0019R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.
Mouse kidney lysates probed with Osteopontin Polyclonal Antibody, unconjugated (bs-0019R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.
Mouse kidney lysates probed with Osteopontin Polyclonal Antibody, unconjugated (bs-0019R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.

Osteopontin Polyclonal Antibody

BS-0019R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetSPP1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    Osteopontin Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID6696
  • Target name
    SPP1
  • Target description
    secreted phosphoprotein 1
  • Target synonyms
    BNSP; BSPI; early T-lymphocyte activation 1; ETA-1; nephropontin; OPN; osteopontin; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1); secreted phosphoprotein 1 variant 6; SPP1/CALPHA1 fusion; urinary stone protein; uropontin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP10451
  • Protein Name
    Osteopontin
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • The Role of MicroRNA-143-5p in the Differentiation of Dental Pulp Stem Cells into Odontoblasts by Targeting Runx2 via the OPG/RANKL Signaling Pathway. Zhan FL et al., 2018 Jan, J Cell Biochem
    Read more
  • Visfatin Destabilizes Atherosclerotic Plaques in Apolipoprotein E-Deficient Mice. Li B et al., 2016, PLoS One
    Read more

Related products

Product group Antibodies
Assay Genie
TargetSPP1
  • SizePrice
Figure 1. Western blot analysis of Osteopontin using anti-Osteopontin antibody (RP1080). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: human SHG-44 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Osteopontin antigen affinity purified polyclonal antibody (Catalog # RP1080) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Osteopontin at approximately 66,25-55KD. The expected band size for Osteopontin is at 35KD.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ELISA
TargetSPP1
  • SizePrice
Product group Antibodies
Absolute Antibody
ApplicationsELISA, ImmunoHistoChemistry, Neutralisation/Blocking, Other Application
TargetSPP1
  • SizePrice
Anti-Osteopontin Antibody
Product group Antibodies
Antibodies.com
ApplicationsWestern Blot, ELISA
  • SizePrice
Product group Antibodies
RayBiotech
ApplicationsWestern Blot
TargetSPP1
  • SizePrice
Product group Antibodies
MedChem Express
TargetSPP1
  • SizePrice
Western Blot analysis of 453 cells using OPN Polyclonal Antibody
Product group Antibodies
Cusabio
SPP1 AntibodyCSB-PA003599
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSPP1
  • SizePrice
Product group Antibodies
Biomatik
ApplicationsELISA, ImmunoHistoChemistry
TargetSPP1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoPrecipitation, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetSPP1
  • SizePrice
Product group Antibodies
Everest Biotech
ApplicationsWestern Blot, ELISA
TargetSPP1
  • SizePrice